Ata Health leads the organizational sustainability objective under USAID’s One Health Workforce Next Generation initiative, including by serving as key personnel for the five- year initiative. The $85 million initiative aims to build a trans-disciplinary One Health workforce in Africa and Southeast Asia and to strengthen the organizational sustainability of two regional university networks. These two networks comprise national level One Health focused networks of universities in 17 countries. In this role, Ata Health led routine benchmarking of network capacity on the NUPAS and NUPAS PLUS tools and led workshops to facilitate networks to identify and prioritize organizational gaps. Ata Health also coordinated the consortium of partners to provide targeted capacity-building that supported measurable improvement to network financial management and internal controls, procurement, operations, and human resources policies and practices, the formulation of new sub-award management capability and policies, project management, partnership development, resource mobilization, and many other areas. As a result of this work, the networks successfully applied for, and received, direct USAID transition awards. This milestone positions the organizations strongly to become prime recipients of future USAID and other donor awards.